Nelfinavir inhibits proliferation and induces DNA damage in thyroid cancer cells. (Record no. 2171)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02791nam a22003137a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170428s20172017 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1351-0088 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| PMID | 28137980 |
| 245 ## - TITLE STATEMENT | |
| Title | Nelfinavir inhibits proliferation and induces DNA damage in thyroid cancer cells. |
| 251 ## - Source | |
| Source | Endocrine-Related Cancer. 24(3):147-156, 2017 Mar |
| 252 ## - Abbreviated Source | |
| Abbreviated source | Endocr Relat Cancer. 24(3):147-156, 2017 Mar |
| 253 ## - Journal Name | |
| Journal name | Endocrine-related cancer |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Year | 2017 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Manufacturer | FY2017 |
| 266 ## - Date added to catalog | |
| Date added to catalog | 2017-05-06 |
| 520 ## - SUMMARY, ETC. | |
| Abstract | Copyright © 2017 Society for Endocrinology. |
| 520 ## - SUMMARY, ETC. | |
| Abstract | The HIV protease inhibitor Nelfinavir (NFV) inhibits PI3K/AKT and MAPK/ERK signaling pathways, emerging targets in thyroid cancers. We examined the effects of NFV on cancer cells that derived from follicular (FTC), papillary (PTC) and anaplastic (ATC) thyroid cancers. NFV (1-20 micro M) was tested in FTC133, BCPAP and SW1736 cell lines. The effects of NFV on cell proliferation were determined in vitro using real-time microscopy and by flow cytometry. DNA damage, apoptotic cell death and expression of molecular markers of epithelial-mesenchymal transition (EMT) were determined by Western blot and real-time PCR. Real-time imaging demonstrated that NFV (10 micro M) increased the time required for the cell passage through the phases of cell cycle and induced DNA fragmentation. Growth inhibitory effects of NFV were associated with the accumulation of cells in G0/G1 phase, downregulation of cyclin D1 and cyclin-dependent kinase 4 (CDK4). NFV also induced the expression of gammaH2AX and p53BP1 indicating DNA damage. Treatment with NFV (20 micro M) resulted in caspase-3 cleavage in all examined cells. NFV (20 micro M) decreased the levels of total and p-AKT in PTEN-deficient FTC133 cells. NFV had no significant effects on total ERK and p-ERK in BRAF-positive BCPAP and SW1736 cells. NFV had no effects on the expression of EMT markers (Twist, Vimentin, E- and N-Cadherin), but inhibited the migration and decreased the abilities of thyroid cancer cells to survive in non-adherent conditions. We conclude that NFV inhibits proliferation and induces DNA damage in thyroid cancer cell lines. Our in vitro data suggest that NFV has a potential to become a new thyroid cancer therapeutic agent. |
| 546 ## - LANGUAGE NOTE | |
| Language note | English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IN PROCESS -- NOT YET INDEXED |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME | |
| Institution | MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION | |
| Department | Medicine/Endocrinology |
| 657 ## - INDEX TERM--FUNCTION | |
| Medline publication type | Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Local Authors | Bikas, Athanasios |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Local Authors | Burman, Kenneth D |
| 790 ## - Authors | |
| All authors | Bikas A, Burman K, Costello J, Jensen K, Kushchayeva Y, McDaniel D, Patel A, Vasko V |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| DOI | <a href="https://dx.doi.org/10.1530/ERC-16-0568">https://dx.doi.org/10.1530/ERC-16-0568</a> |
| Public note | https://dx.doi.org/10.1530/ERC-16-0568 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| Item type description | Article |
| Withdrawn status | Lost status | Damaged status | Not for loan | Collection | Home library | Current library | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MedStar Authors Catalog | MedStar Authors Catalog | 05/06/2017 | 28137980 | 28137980 | 05/06/2017 | 05/06/2017 | Journal Article |